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Product Info Summary
| SKU: | A05829-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-Gsta3 Antibody Picoband™
SKU/Catalog Number
A05829-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Gsta3 Antibody Picoband™ catalog # A05829-3. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gsta3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05829-3)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Gsta3, which shares 57.9% and 89.5% amino acid (aa) sequence identity with human and rat Gsta3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A05829-3 is reactive to GSTA3 in Mouse, Rat
Applications
A05829-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
28 kDa
Calculated molecular weight
25.302kDa
Background of GSTA3
Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes are involved in cellular defense against toxic, carcinogenic, and pharmacologically active electrophilic compounds. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class genes that are located in a cluster mapped to chromosome 6. Genes of the alpha class are highly related and encode enzymes with glutathione peroxidase activity. However, during evolution, this alpha class gene diverged accumulating mutations in the active site that resulted in differences in substrate specificity and catalytic activity. The enzyme encoded by this gene catalyzes the double bond isomerization of precursors for progesterone and testosterone during the biosynthesis of steroid hormones. An additional transcript variant has been identified, but its full length sequence has not been determined.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Rat
Flow Cytometry, 1-3μg/1x106 cells, Mouse
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Gsta3 using anti-Gsta3 antibody (A05829-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: rat testis tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gsta3 antigen affinity purified polyclonal antibody (Catalog # A05829-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gsta3 at approximately 28KD. The expected band size for Gsta3 is at 28KD.
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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Gsta3 antibody (A05829-3).
Overlay histogram showing HEPA1-6 cells stained with A05829-3 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Gsta3 Antibody (A05829-3, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For GSTA3 (Source: Uniprot.org, NCBI)
Gene Name
GSTA3
Full Name
Glutathione S-transferase A3
Weight
25.302kDa
Superfamily
GST superfamily
Alternative Names
EC 2.5.1.18; glutathione S-alkyltransferase A3; glutathione S-aralkyltransferase A3; glutathione S-aryltransferase A3; glutathione S-transferase A3; Glutathione S-transferase A3-3; glutathione S-transferase alpha 3; GST class-alpha member 3; GSTA3-3; GTA3; MGC22232; S-(hydroxyalkyl)glutathione lyase A3 GSTA3 GSTA3-3, GTA3 glutathione S-transferase alpha 3 glutathione S-transferase A3|GST class-alpha member 3|S-(hydroxyalkyl)glutathione lyase A3|glutathione S-alkyltransferase A3|glutathione S-aralkyltransferase A3|glutathione S-aryltransferase A3|glutathione S-transferase A3-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSTA3, check out the GSTA3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSTA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Gsta3 Antibody Picoband™ (A05829-3)
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No publications found for A05829-3
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